Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino
文献类型：CNKI期刊论文
| 作者 | Kong N(孔宁) ; Liu X(刘晓); Li JY(李俊元); Mu WD(穆文丹); Lian JW(连建武); Xue YJ(薛艳洁); Li Q(李琪)
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| 发表日期 | 2017-09-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Pacific abalone temperature salinity survival growth DNA methylation |
| 英文摘要 | Temperature and salinity are two of the most potent abiotic factors infl uencing marine mollusks.In this study,we investigated the individual and combined ef fects of temperature and salinity on the survival and growth of juvenile Pacifi c abalone,Haliotis discus hannai Ino,and also examined the DNA methylation alteration that may underpin the phenotypic variation of abalone exposed to dif ferent rearing conditions.The single-factor data showed that the suitable ranges of temperature and salinity were 16–28°C at a constant salinity of 32,and 24–40 at a constant temperature of 20°C,respectively.The two-factor data indicated that both survival and growth were signifi cantly af fected by temperature,salinity and their interaction.The optimal temperature-salinity combination for juveniles was 23–25°C and 30–36.To explore environment-induced DNA methylation alteration,the methylation-sensitive amplifi ed polymorphism(MSAP)technique was used to analyze the genomic methylation profi les of abalone reared in optimal and adverse conditions.Neither temperature nor salinity induced evident changes in the global methylation level,but 67 and 63 dif ferentially methylated loci were identifi ed in temperature and salinity treatments,respectively.The between-group eigen analysis also showed that both temperature and salinity could induce epigenetic dif ferentiation in H.discus hannai Ino.The results of our study provide optimal rearing conditions for juvenile H.discus hannai Ino,and represent the fi rst step toward revealing the epigenetic regulatory mechanism of abalone in response to thermal and salt stresses. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412) |
| 卷 | v.35期:05页:264-274 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189186]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.NingboUniversity 4.OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Kong N,Liu X,Li JY,et al. Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino. 2017. |
入库方式： OAI收割
来源：海洋研究所
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