Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis:identification of developmental genes at the earliest stages
文献类型：CNKI期刊论文
| 作者 | Hui M(惠敏); Cui CX(崔朝霞); Liu Y(刘媛); Song CW(宋呈文)
|
| 发表日期 | 2017-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | mitten crabs embryo development fertilized egg cleavage transcriptomics |
| 英文摘要 | In crab,embryogenesis is a complicated developmental program marked by a series of critical events.RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever before.Here,we present a comprehensive analysis of the transcriptomes of Eriocheir sinensis oosperms(Os) and embryos at the 2-4 cell stage(Cs),which are separated by a cleavage event.A total of 18 923 unigenes were identified,and 403 genes matched with gene ontology(GO)terms related to developmental processes.In total,432 differentially expressed genes(DEGs) were detected between the two stages.Nine DEGs were specifically expressed at only one stage.These DEGs may be relevant to stage-specific molecular events during development.A number of DEGs related to 'hedgehog signaling pathway','Wnt signaling pathway' 'germplasm','nervous system','sensory perception' and'segment polarity' were identified as being up-regulated at the Cs stage.The results suggest that these embryonic developmental events begin before the early cleavage event in crabs,and that many of the genes expressed in the two transcriptomes might be maternal genes.Our study provides ample information for further research on the molecular mechanisms underlying crab development. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A409) ; the Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02) ; the National Natural Science Foundation of China(Nos.41276165 to Dr.CUI Zhaoxia,31302187 to Dr.HUI Min) |
| 卷 | v.35期:04页:50-61 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189246]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.National&LocalJointEngineeringLaboratoryofEcologicalMariculture 4.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Hui M,Cui CX,Liu Y,et al. Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis:identification of developmental genes at the earliest stages. 2017. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。