Effects of Lugol's iodine solution and formalin on cell volume of three bloom-forming dinoflagellates
文献类型：CNKI期刊论文
| 作者 | Yang Y(杨洋) ; Sun XX(孙晓霞); Zhao YF(赵永芳)
|
| 发表日期 | 2017-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | cell volume formalin Lugol's Noctiluca scintillans |
| 英文摘要 | Fixatives are traditionally used in marine ecosystem research.The bias introduced by fixatives on the dimensions of plankton cells may lead to an overestimation or underestimation of the carbon biomass.To determine the impact of traditional fixatives on dinoflagellates during short-and long-term fixation,we analyzed the degree of change in three bloom-forming dinoflagellates(Prorocentrum micans,Scrippsiella trochoidea and Noctiluca scintillans) brought about by Lugol's iodine solution(hereafter Lugol's) and formalin.The fixation effects were species-specific.P.micans cell volume showed no significant change following long-term preservation,and S.trochoidea swelled by approximately 8.06% in Lugol's and by 20.97% in formalin as a percentage of the live cell volume,respectively.N.scintillans shrank significantly in both fixatives.The volume change due to formalin in N.scintillans was not concentration-dependent,whereas the volume shrinkage of N.scintillans cells fixed with Lugol's at a concentration of 2% was nearly six-fold that in cells fixed with Lugol's at a concentration of 0.6%-0.8%.To better estimate the volume of N.scintillans fixed in formalin at a concentration of 5%,we suggest that the conversion relationship was as follows:volume of live cell=volume of intact fixed cell/0.61.Apart from size change,damage induced by fixatives on N.scintillans was obvious.Lugol's is not a suitable fixative for N.scintillans due to high frequency of broken cells.Accurate carbon biomass estimate of N.scintillans should be performed on live samples.These findings help to improve the estimate of phytoplankton cell volume and carbon biomass in marine ecosystem. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China(973Program)(No.2014CB441504) ; the"Strategic Priority Research ProgramWestern Pacific Ocean System"of the Chinese Academy of Sciences(No.XDA11030204) ; the Key Program of the National Natural Science Foundation of China(No.41230963) ; the NSFC-Shandong Province Joint Fund Project(No.U1406403) |
| 卷 | v.35期:04页:138-146 |
| 语种 | 英文; |
| 分类号 | X145;X173 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189248]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.UniversityofChineseAcademyofSciences 2.JiaozhouBayMarineEcosystemResearchStation,InstituteofOceanology,ChineseAcademyofSciences 3.LaboratoryforMarineEcologyandEnvironmentalScience,QingdaoNationalLaboratoryforMarineScienceandTechnology |
| 推荐引用方式 GB/T 7714 | Yang Y,Sun XX,Zhao YF. Effects of Lugol's iodine solution and formalin on cell volume of three bloom-forming dinoflagellates. 2017. |
入库方式： OAI收割
来源：海洋研究所
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