Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48
文献类型：CNKI期刊论文
| 作者 | Xu R(徐蕊) ; Li XM(李晓明); Xu GM(徐刚明); Wang BG(王斌贵)
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| 发表日期 | 2017-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Aspergillus wentii asperolide A anti-tumor agent fermentation optimization |
| 英文摘要 | The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A, a tetranorlabdane diterpenoid active against lung cancer. However, the fermentation yield of asperolide A was very low and only produced in static cultures. Static fermentation conditions of A. wentii EN-48 were optimized employing response surface methodology to enhance the production of asperolide A. The optimized conditions resulted in a 13.9-fold yield enhancement, which matched the predicted value, and the optimized conditions were successfully used in scale-up fermentation for the production of asperolide A. Exogenous addition of plant hormones(especially 10 μmol/L methyl jasmonate) stimulated asperolide A production. To our knowledge, this is first optimized production of an asperolide by a marine-derived fungus. The optimization is effective and valuable to supply material for further anti-tumor mechanism studies and preclinical evaluation of asperolide A and other norditerpenoids. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(NSFC,Nos.31200042,31570038) ; the NSFC-Shandong Joint Fund for Marine Science Research Centers(No.U1406402) |
| 卷 | v.35期:03页:192-197 |
| 语种 | 英文; |
| 分类号 | R915 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189315]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryofMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology,KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Xu R,Li XM,Xu GM,et al. Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48. 2017. |
入库方式： OAI收割
来源：海洋研究所
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