Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
文献类型：CNKI期刊论文
| 作者 | Liu M(刘盟); Liu Y(刘媛); Hui M(惠敏); Song CW(宋呈文) ; Cui CX(崔朝霞)
|
| 发表日期 | 2017-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Portunus trituberculatus clip domain serine proteinase serine proteinase homolog polymorphism susceptibility/resistance |
| 英文摘要 | Clip domain serine proteases(cSPs) and their homologs(SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades,especially in the innate immune responses of invertebrates.Here,polymorphisms of PtcSP and PtSPH from the swimming crab Portunus trituberculatus were investigated to explore their association with resistance/susceptibility to Vibrio alginolyticus.Polymorphic loci were identified using Clustal X,and characterized with SPSS 16.0 software,and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a χ~2 test.A total of 109 and 77 single nucleotide polymorphisms(SNPs) were identified in the genomic fragments of PtcSP and PtSPH,respectively.Notably,nearly half of PtSPH polymorphisms were found in the non-coding exon 1.Fourteen SNPs investigated were significantly associated with susceptibility/resistance to V.alginolyticus(P<0.05).Among them,eight SNPs were observed in introns,and one synonymous,four non-synonymous SNPs and one ins-del were found in coding exons.In addition,five simple sequence repeats(SSRs) were detected in intron 3 of PtcSP.Although there was no statistically significant difference of allele frequencies,the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks.After further validation,polymorphisms investigated here might be applied to select potential molecular markers of P.trituberculatus with resistance to V.alginolyticus. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(Nos.41206147,31302187) ; the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02) |
| 卷 | v.35期:02页:13-21 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189369]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 2.CollegeofFisheriesandLifeScience,DalianOceanUniversity 3.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 4.National&LocalJointEngineeringLaboratoryofEcologicalMariculture |
| 推荐引用方式 GB/T 7714 | Liu M,Liu Y,Hui M,et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus. 2017. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。