Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus
文献类型：CNKI期刊论文
| 作者 | Peng LM(彭丽敏) ; Zheng Y(郑媛); You F(尤锋); Wu ZH(吴志昊); Zou YX(邹玉霞); Zhang PJ(张培军)
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| 发表日期 | 2016-09-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | olive flounder Paralichthys olivaceus testis Sertoli cell chromosome FasL transfection |
| 英文摘要 | The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis.A new Sertoli cell line(POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages.Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P.olivaceus.Cells were optimally maintained at 25℃ in DMEM/F12 medium supplemented with fetal bovine serum,basic fibroblast growth factor,and epidermal growth factor.The growth curve of POSC showed a typical "S" shape.Chromosome analysis revealed that the cell line possessed the normal P.olivaceus diploid karyotype of 2n=48t.POSC expressed dmrt1 but not vasa,which was detected using RT-PCR and sequencing.Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL.Therefore,POSC appeared to consist of testicular Sertoli cells.Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid,with the transfection efficiency reaching 10%.This research not only offers an ideal model for further gene expression and regulation studies on P.olivaceus,but also serves as valuable material in studying fish spermatogenesis,Sertoli cell-germ cell interactions,and the mechanism of growth and development of testis. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2012AA10A408,2012AA10A402) ; the National Natural Science Foundation of China(No.41276171) ; the National Flatfish Industry System Construction Program(No.nycytx-50-G03) |
| 卷 | v.34期:05页:172-181 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189552]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 3.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Peng LM,Zheng Y,You F,et al. Establishment and characterization of a testicular Sertoli cell line from olive flounder Paralichthys olivaceus. 2016. |
入库方式： OAI收割
来源：海洋研究所
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