Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus
文献类型：CNKI期刊论文
| 作者 | JI Liqin; JIANG Keyong; LIU Mei; WANG Baojie; HAN Longjiang; ZHANG Mingming; WANG Lei |
| 发表日期 | 2016-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | hematology HSP70 HSP90 mRNA expression low temperature stress fish |
| 英文摘要 | To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot( Scophthalmus maximus),water temperature was lowered rapidly from 18 to 1°C.During the cooling process,three individuals were removed from culture tanks at 18,13,8,5,3,and 1°C.Blood samples and tissues were taken from each individual,hematological indices and HSP gene expression in tissues were measured.The red blood cell count,white blood cell count,and hemoglobin concentration decreased significantly( P < 0.05) as temperature decreased.Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased,whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1°C and lactate dehydrogenase activity increased first and then decreased.Blood urea nitrogen and uric acid levels were highest at 8°C,and creatinine concentration was highest at 3°C.The concentrations of plasma cortisol,cholesterol,and triglyceride all increased significantly( P < 0.05) as temperature decreased.The serum glucose concentration increased first and then decreased to the initial level.The HSP70 m RNA expression showed various patterns in different tissues,whereas HSP90 m RNA expression showed the same tendency in all tissues.Overall,these results indicate that temperature decreases in the range of 8 to 5°C may induce a stress response in S.maximus and that temperature should be kept above 8°C in the aquaculture setting to avoid damage to the fish. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Special Foundation for Youth Science and Technology of Qingdao(No.14-2-4-59-jch) ; the Science and Technology Development Planning of Shandong Province(No.2011GGC21007) ; the National Natural Science Foundation of China(No.41206144) |
| 卷 | v.34期:03页:10-20 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189687]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.FisheriesCollege,OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | JI Liqin,JIANG Keyong,LIU Mei,et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus. 2016. |
入库方式： OAI收割
来源：海洋研究所
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