Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica
文献类型：CNKI期刊论文
| 作者 | Zhang WJ(张文静) ; Sun DL(孙德林); Zhao X(赵峡); Jin WH(金维华); Wang J(王晶); Zhang QB(张全斌)
|
| 发表日期 | 2016-01-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | sulfated polysaccharide HPLC post-column derivatization microanalysis pharmacokinetics |
| 英文摘要 | A rapid,sensitive and reproducible high performance liquid chromatography(HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecularweight sulfated polysaccharide(GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection,and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase(t_(1/2α))=11.24±2.93 min,half-time of elimination phase(t_(1/2β))=98.20±25.78 min,maximum concentration(C_(max))=110.53 μg/mL and peak time(T_(max))=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However,the concentration of GFS found in serum was too low for detection,and GFS could only be detected for up to 2 h after intragastric administration(200 mg/kg body weight). Thus,the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion,HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.41376166) ; the Ocean Public Welfare Scientific Research Project(Nos.201005024,201405040) ; the Jiangsu Science and Technology Project(No.BE2012687) ; the Special Fund for Cooperation between Jilin Province and Chinese Academy of Sciences(No.2013SYHZ0023) |
| 卷 | v.34期:01页:179-187 |
| 语种 | 英文; |
| 分类号 | R914;R96 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189805]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences 3.HezeJunxinyuanFoodCompany 4.OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Zhang WJ,Sun DL,Zhao X,et al. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica. 2016. |
入库方式： OAI收割
来源：海洋研究所
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