Isolation, Expression and Characterization of rbc L Gene from Ulva prolifera J. Agardh(Ulvophyceae, Chlorophyta)
文献类型：CNKI期刊论文
| 作者 | SHAO Zhanru; LI Wei; GUO Hui; DUAN Delin |
| 发表日期 | 2015-12-06 |
| 出处 | Journal of Ocean University of China
 |
| 关键词 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit sequence analysis real-time PCR in vitro expres-sion Ulva prolifera |
| 英文摘要 | Ulva prolifera is a typical green alga in subtidal areas and can grow tremendously fast. A highly efficient Rubisco enzyme which is encoded by Up Rbc L gene may contribute to the rapid growth. In this study, the full-length Up Rbc L open reading frame(ORF) was identified, which encoded a protein of 474 amino acids. Phylogenetic analysis of Up Rbc L sequences revealed that Chlorophyta had a closer genetic relationship with higher plants than with Rhodophyta and Phaeophyta. The two distinct residues(aa11 and aa91) were presumed to be unique for Rubisco catalytic activity. The predicted three-dimensional structure showed that one α/β-barrel existed in the C-terminal region, and the sites for Mg~(2+)coordination and CO_2 fixation were also located in this region. Gene expression profile indicated that Up Rbc L was expressed at a higher level under light exposure than in darkness. When the culture temperature reached 35℃, the expression level of Up Rbc L was 2.5-fold lower than at 15℃, and the carboxylase activity exhibited 13.8-fold decrease. Up Rbc L was heterologously expressed in E. coli and was purified by Ni~(2+) affinity chromatography. The physiological and biochemical characterization of recombinant Rubisco will be explored in the future. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | financially supported by the Scientific and Technical Supporting Programs of China(2008 BAC49B01) ; State Ocean Administration Project(200805069) |
| 卷 | v.14期:06页:161-169 |
| 语种 | 英文; |
| 分类号 | S917.3 |
| ISSN号 | 1672-5182 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189856]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.UniversityofChineseAcademyofSciences 2.InstituteofOceanology,ChineseAcademyofSciences 3.InstituteofGeneralBotanyandPlantPhysiology,FriedrichSchillerUniversity |
| 推荐引用方式 GB/T 7714 | SHAO Zhanru,LI Wei,GUO Hui,et al. Isolation, Expression and Characterization of rbc L Gene from Ulva prolifera J. Agardh(Ulvophyceae, Chlorophyta). 2015. |
入库方式： OAI收割
来源：海洋研究所
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