Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome
文献类型：CNKI期刊论文
| 作者 | Zhang H(张好); Li FC(李富超); Chen HX(陈华新); Zhao J(赵瑾); Yan JF(闫晋飞); Jiang P(姜鹏); Li RG(李荣贵); Zhu BL(朱宝利) |
| 发表日期 | 2015-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | marine sediment metagenome lipolytic expression esterase characterization |
| 英文摘要 | Lipolytic enzymes, including esterases and lipases, represent a group of hydrolases that catalyze the cleavage and formation of ester bonds. A novel esterase gene, scsEst01, was cloned from a South China Sea sediment metagenome. The scsEst01 gene consisted of 921 bp encoding 307 amino acid residues. The predicted amino acid sequence shared less than 90% identity with other lipolytic enzymes in the NCBI nonredundant protein database. Scs Est01 was successfully co-expressed in E scherichia coli BL21(DE3) with chaperones(dnaK-dna J-grp E) to prevent the formation of inclusion bodies. The recombinant protein was purified on an immobilized metal ion affinity column containing chelating Sepharose charged with Ni2 +. The enzyme was characterized using p-nitrophenol butyrate as a substrate. Scs Est01 had the highest lipolytic activity at 35℃ and p H 8.0, indicative of a meso-thermophilic alkaline esterase. Scs Est01 was thermostable at 20℃. The lipolytic activity of scs Est01 was strongly increased by Fe2 +, Mn 2+ and 1% Tween 80 or Tween 20. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11030404) ; the National High Technology Research and Development Program of China(863 Program)(Nos.2012AA092103,2014AA093501,2014AA093505) ; the Knowledge Innovation Program of Chinese Academy of Sciences(No.KZCX2-YW-JC201) ; the Open Project Program of Key Laboratory of Marine Bio-Resources Sustainable Utilization,South China Sea Institute of Oceanology,Chinese Academy of Sciences(No.LMB121006) |
| 卷 | v.33期:04页:3-11 |
| 语种 | 英文; |
| 分类号 | Q55 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189981]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.LaboratoryofMicrobiology,ShandongProvince,QingdaoUniversity 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.ThirdInstituteofOceanography,StateOceanicAdministration 4.KeyLaboratoryofPathogenicMicrobiologyandImmunology,InstituteofMicrobiology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Zhang H,Li FC,Chen HX,et al. Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome. 2015. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。