Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks
文献类型：CNKI期刊论文
| 作者 | Huang W(黄雯) ; Xu F(许飞); Qu T(曲涛); Li L(李莉); Que HY(阙华勇); Zhang GF(张国范)
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| 发表日期 | 2015-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | iodothyronine deiodinase C rassostrea gigas thyroid hormone(TH) clone expression analysis |
| 英文摘要 | Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, only one deiodinase has been identified in the scallop C hlamys farreri. Here, two deiodinases were cloned in the Pacific oyster C rassostrea gigas( Cg Dx and C g Dy). The characteristic in-frame TGA codons and selenocysteine insertion sequence elements in the oyster deiodinase c DNAs supported the activity of them. Furthermore, seven orthologs of deiodinases were found by a tblastn search in the mollusk Lottia gigantea and the annelid C apitella teleta. A phylogenetic analysis revealed that the deiodinase gene originated from an common ancestor and a clade-specific gene duplication occurred independently during the differentiation of the mollusk, annelid, and vertebrate lineages. The distinct spatiotemporal expression patterns implied functional divergence of the two deiodinases. The expression of C g Dx and Cg Dy was influenced by L-thyroxine T4, and putative thyroid hormone responsive elements were found in their promoters, which suggested that the oyster deiodinases were feedback regulated by TH. Epinephrine stimulated the expression level of C g Dx and Cg Dy, suggesting an interaction effect between different hormones. This study provides the first evidence for the existence of a conserved TH feedback regulation mechanism in mollusks, providing insights into TH evolution. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(Nos.31372515,31402285) ; the National Basic Research Program of China(973 Program)(No.2010CB126401) ; the National High Technology Research and Development Program(863 Program)(No.2012AA10A405) |
| 卷 | v.33期:04页:181-190 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/189984]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.National&LocalJointEngineeringLaboratoryofEcologicalMariculture,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Huang W,Xu F,Qu T,et al. Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks. 2015. |
入库方式： OAI收割
来源：海洋研究所
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