Expression and enzymatic characterization of a cold-adapted β-agarase from Antarctic bacterium Pseudoalteromonas sp.NJ21
文献类型：CNKI期刊论文
| 作者 | Li J(李江); Sha YJ(沙玉杰)
|
| 发表日期 | 2015-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Antarctic bacterium Pseudoalteromonas agarase expression enzymatic characterization |
| 英文摘要 | An agar-degrading bacterium,designated as Pseudoalteromonas sp. NJ21,was isolated from an Antarctic sediment sample. The agarase gene a ga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase(r Aga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30–40°C and 8.0,respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10°C,which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an β-agarase,producing neoagarobiose(NA2) as the final main product. Furthermore,this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine,food and cosmetic industries. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Public Science and Technology Research Funds Project of Ocean(No.201105027) ; the Shandong Province Young and the Middle-Aged Scientists Research Awards Fund(No.DS2010HZ001) ; the Basic Scientific Research Funds of First Institute of Oceanography,State Oceanic Administration(No.GY02-2011G17) |
| 卷 | v.33期:02页:31-39 |
| 语种 | 英文; |
| 分类号 | S917.1 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190095]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLabofMarineBioactiveSubstances,FirstInstituteofOceanography,StateOceanicAdministration(SOA) 2.InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Li J,Sha YJ. Expression and enzymatic characterization of a cold-adapted β-agarase from Antarctic bacterium Pseudoalteromonas sp.NJ21. 2015. |
入库方式： OAI收割
来源：海洋研究所
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