Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate
文献类型：CNKI期刊论文
| 作者 | Wang XQ(王雪芹); Xing RE(邢荣娥) ; Liu S(刘松); Yu HH(于华华); Li KC(李克成); Chen ZY(陈作元); Li PC(李鹏程)
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| 发表日期 | 2015-01-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | mackerel protein antioxidant activity fraction of hydrolysate ultrafiltration purification |
| 英文摘要 | Mackerel(Pneumatophorus japonicus) proteins were hydrolyzed by five proteases:trypsin,papain,neutrase,acid protease,and flavourzyme.The hydrolysate treated by neutrase exhibited the highest antioxidant activity.Response surface methodology(RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate(MPH) with the highest DPPH radical scavenging activity.The MPH was fractioned using a series of ultrafiltration membranes and five fractions,namely,MPH-Ⅰ(>10kDa),MPH-Ⅱ(10-2.5 kDa),MPH-Ⅲ(1-2.5 kDa),MPH-Ⅳ(0.4-1 kDa),and MPH-V(below 0.4 kDa),were obtained.DPPH radical scavenging activity,reducing power,hydroxyl radical scavenging activity,and the lipid peroxidation inhibition capability of these fractions were evaluated.The fractions in molecular weights <2.5 kDa(MPH-Ⅲ,MPH-Ⅳ,and MPH-Ⅴ),which occupied 93.4%of the total fractions,showed the strongest antioxidant activity;and the antioxidant activities of the three fractions are similar to each other.Using SP Sephadex C-25 and Sephadex G-25 columns,eight fractions were obtained from the MPH(<2.5 kDa).The isolated peptide I(1 664 kDa) displayed the highest DPPH radical scavenging activity.Therefore,MPH is a potential source of antioxidant peptides. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Commonweal Item of State Oceanic Administration of the People's Republic of China(Nos.201305016-2,201405038-2) |
| 卷 | v.33期:01页:161-170 |
| 语种 | 英文; |
| 分类号 | TQ464 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190166]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.DepartmentofCardiology,theAffiliatedHospitalofQingdaoUniversityMedicalCollege 2.InstituteofOceanology,ChineseAcademyofSciences 3.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Wang XQ,Xing RE,Liu S,et al. Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate. 2015. |
入库方式： OAI收割
来源：海洋研究所
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