Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei
文献类型：CNKI期刊论文
| 作者 | Yu Y(于洋) ; Zhang XJ(张晓军); Liu JW(刘敬文); Li FH(李富花); Huang H(黄皓); Li YJ(李义军); Liu XL(刘小林); Xiang JH(相建海)
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| 发表日期 | 2015-01-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | molecular identification Litopenaeus vannamei new variety mitochondrial control region microsatellite |
| 英文摘要 | Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance.However,the identification of new varieties of shrimps from their phenotypic characters is difficult.This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences.The method was employed to identify a new selected variety,Kehai No.1(KH-1),from three representative stocks(control group):Zhengda;Tongwei;and a stock collected from Fujian Province,which is now cultured in China's mainland.By pooled genotyping of KH-1 and the control group,five microsatellites showing differences between KH-1 and the control group were screened out.Individual genotyping data confirmed the results from pooled genotyping.The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2.By sequencing the mitochondrial control regions of individuals from the KH-1 and control group,four haplotypes were observed in the KH-1 group,whereas14 haplotypes were obtained in the control group.By combining the microsatellite assignment analysis with mitochondrial control region analysis,the average accuracy of identification of individuals in the KH-1group and control group reached 89%.The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L.vannamei from other populations cultured in China. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2012AA10A404,2012AA092205) |
| 卷 | v.33期:01页:3-12 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190186]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.HainanNanjiangMarineBiotechniqueCompanyLimited 2.UniversityofChineseAcademyofSciences 3.NorthwestAgricultureandForestryUniversity 4.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Yu Y,Zhang XJ,Liu JW,et al. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei. 2015. |
入库方式： OAI收割
来源：海洋研究所
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