Profiling and Comparison of Color Body Wall Transcriptome of Normal Juvenile Sea Cucumber(Apostichopus japonicus) and Those Produced by Crossing Albino
文献类型：CNKI期刊论文
| 作者 | MA Deyou; YANG Hongsheng ; SUN Lina
|
| 发表日期 | 2014-12-06 |
| 出处 | Journal of Ocean University of China
 |
| 关键词 | Apostichopus japonicus sezparation gene expression profiling RNA-seq real-time PCR |
| 英文摘要 | Sea cucumber(Apostichopus japonicus) is one of the most important aquaculture animals in China. Usually its normal body color is black that fits its living environment. The juvenile individuals obtained by crossing albino sea cucumber segregated in body color. To document the transcriptome difference between albino associating sea cucumber and the control, we sequenced their transcriptomes with RNA-seq. Approximately, 4.790 million(M) and 4.884 M reads, 200 nt in length, were generated from the body wall of albino associating sea cucumber and the control, respectively, from them, 9550(46.81%) putative genes were identified. In total, 583 genes were found to express differentially between albino associating sea cucumber and the control. Of these differentially expressed genes(DEGs), 4.8% changed more than five-folds. The expression levels of eight DEGs were confirmed with real-time PCR. The changing trend of these DEGs detected with real-time PCR agreed well with that detected with RNA-seq, although the change degree of some DEGs was different. Four significantly enriched pathways were identified for DEGs, which included phagocytosis, Staphylococcus aureus infection, ECM-receptor interaction and focal adhesion. These pathways were helpful for understanding the physiological difference between albino associating sea cucumber and the control. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | funded by the National Natural Science Foundation of China (No. 40976089) ; the National Key Technology Support Program of China (No. 2011BAD13B02) ; the National Oceanic Public Welfare Industry Special Scientific Research of China (No. 201205023) ; the Chinese National 863 Project (2012AA10A412) |
| 卷 | v.13期:06页:155-164 |
| 语种 | 英文; |
| 分类号 | S968.9 |
| ISSN号 | 1672-5182 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190224]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences(IOCAS) 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | MA Deyou,YANG Hongsheng,SUN Lina. Profiling and Comparison of Color Body Wall Transcriptome of Normal Juvenile Sea Cucumber(Apostichopus japonicus) and Those Produced by Crossing Albino. 2014. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。