Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation
文献类型：CNKI期刊论文
| 作者 | Zhao Y(赵业) ; Chen MY(陈慕雁); Wang TM(王天明); Sun LN(孙丽娜); Xu DX(徐冬雪); Yang HS(杨红生)
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| 发表日期 | 2014-11-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | Apostichopus japonicus sea cucumber aestivation quantitative real-time RT-PCR reference gene normalization factor |
| 英文摘要 | Quantitative real-time reverse transcription-polymerase chain reaction(qRT-PCR) is a technique that is widely used for gene expression analysis,and its accuracy depends on the expression stability of the internal reference genes used as normalization factors.However,many applications of qRT-PCR used housekeeping genes as internal controls without validation.In this study,the expression stability of eight candidate reference genes in three tissues(intestine,respiratory tree,and muscle) of the sea cucumber Apostichopus japonicus was assessed during normal growth and aestivation using the geNorm,NormFinder,delta CT,and RefFinder algorithms.The results indicate that the reference genes exhibited significantly different expression patterns among the three tissues during aestivation.In general,the P-tubulin(TUBB) gene was relatively stable in the intestine and respiratory tree tissues.The optimal reference gene combination for intestine was 40 S ribosomal protein S18(RPS18),TUBB,and NADH dehydrogenase(NADH);for respiratory tree,it was P-actin(ACTB),TUBB,and succinate dehydrogenase cytochrome B small subunit(SDHC);and for muscle it was a-tubulin(TUBA) and NADH dehydrogenase[ubiquinone]1 a subcomplex subunit 13(NDUFA13).These combinations of internal control genes should be considered for use in further studies of gene expression in A.japonicus during aestivation. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Nature Science Foundation of China(Nos.41176139,31201972) |
| 卷 | v.32期:06页:51-59 |
| 语种 | 英文; |
| 分类号 | S917.4;Q78 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190244]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.OceanUniversityofChina 2.InstituteofOceanology,ChineseAcademyofSciences 3.UniversityofChineseAcademyofSciences 4.ZhejiangOceanUniversity |
| 推荐引用方式 GB/T 7714 | Zhao Y,Chen MY,Wang TM,et al. Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation. 2014. |
入库方式： OAI收割
来源：海洋研究所
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