A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance
文献类型：CNKI期刊论文
| 作者 | Liu JW(刘敬文); Yu Y(于洋) ; Li FH(李富花); Zhang XJ(张晓军); Xiang JH(相建海)
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| 发表日期 | 2014-11-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | anti-lipopolysaccharide factors(ALFs) single nucleotide polymorphisms(SNPs) white spot syndrome virus(WSSV) resistance Litopenaeus vannamei |
| 英文摘要 | Anti-lipopolysaccharide factors(ALFs) are basic components of the crustacean immune system that defend against a range of pathogens.The cDNA sequence of a new ALF,designated nLvALF2,with an open reading frame encoding 132 amino acids was cloned.Its deduced amino acid sequence contained the conserved functional domain of ALFs,the LPS binding domain(LBD).Its genomic sequence consisted of three exons and four introns.nLvALF2 was mainly expressed in the Oka organ and gills of shrimps.The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus(WSSV) infection,suggesting its important roles in protecting shrimps from WSSV.Single nucleotide polymorphisms(SNPs)were found in the genomic sequence of nLvALF2,of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV.The loci g.2422 A>G,g.2466 T>C,and g.2529 G>A were significantly associated with the resistance to WSSV(P<0.05).These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2012AA10A404,2012AA092205) ; the General Program of National Natural Science Foundation of China(Nos.31272683,31072203)to Dr.LI Fuhua |
| 卷 | v.32期:06页:35-50 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190254]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.National&LocalJointEngineeringLaboratoryofEcologicalMariculture,InstituteofOceanology,ChineseAcademyofSciences 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Liu JW,Yu Y,Li FH,et al. A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance. 2014. |
入库方式： OAI收割
来源：海洋研究所
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