Biological identification and determination of optimum growth conditions for four species of Navicula
文献类型：CNKI期刊论文
| 作者 | ZHAO Xiaobo; PANG Shaojun; LIU Feng; SHAN Tifeng; LI Jing
|
| 发表日期 | 2014-08-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | Navicula,SSU,rbcL,culture conditions |
| 英文摘要 | Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy(SEM) and sequence comparisons of two segments of genes(small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm(OD680) under various environmental factors. Results showed that the optimum culture conditions were 25℃, 50–100 μmol photons m-2 s-1, pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO32-. Their relative growth rates(RGR) peaked at the highest tested level(0.25 mmol/L). The optimal concentrations of NO3- and PO43- were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The National Natural Science Foundation of China under contract No.41176135 |
| 卷 | v.33期:08页:113-120 |
| 语种 | 英文; |
| 分类号 | S968.49 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190350]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.AlgalCultureCollectionCentre,KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | ZHAO Xiaobo,PANG Shaojun,LIU Feng,et al. Biological identification and determination of optimum growth conditions for four species of Navicula. 2014. |
入库方式： OAI收割
来源：海洋研究所
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