Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics
文献类型：CNKI期刊论文
| 作者 | Cheng FP(程方平); Wang MX(王敏晓); Li CL(李超伦) ; Sun S(孙松)
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| 发表日期 | 2014-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | zooplankton DNA barcodes cytochrome oxidase subunit 1(cox1) |
| 英文摘要 | DNA barcoding provides accurate identification of zooplankton species through all life stages. Single-gene-targeted metagenomic analysis based on DNA barcode databases can facilitate longterm monitoring of zooplankton communities. With the help of the available zooplankton databases, the zooplankton community of the Changjiang(Yangtze) River estuary was studied using a single-gene-targeted metagenomic method to estimate the species richness of this community. A total of 856 mitochondrial cytochrome oxidase subunit 1(cox1) gene sequences were determined. The environmental barcodes were clustered into 70 molecular operational taxonomic units(MOTUs). Forty-two MOTUs matched barcoded marine organisms with more than 90% similarity and were assigned to either the species(similarity>96%) or genus level(similarity<96%). Sibling species could also be distinguished. Many species that were overlooked by morphological methods were identified by molecular methods, especially gelatinous zooplankton and merozooplankton that were likely sampled at different life history phases. Zooplankton community structures differed significantly among all of the samples. The MOTU spatial distributions were infl uenced by the ecological habits of the corresponding species. In conclusion, single-gene-targeted metagenomic analysis is a useful tool for zooplankton studies, with which specimens from all life history stages can be identified quickly and effectively with a comprehensive database. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.41230963) ; the National Basic Research Program of China(973 Program)(No.2011CB403604) ; the“135”Fund of Institute of Oceanology,Chinese Academy of Sciences(No.2012I0060102) ; the Innovative Research Group Funding of the National Natural Science Foundation of China(No.41121064) ; the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11020305) |
| 卷 | v.32期:04页:124-136 |
| 语种 | 英文; |
| 分类号 | Q958 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190390]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.UniversityofChineseAcademyofSciences 2.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Cheng FP,Wang MX,Li CL,et al. Zooplankton community analysis in the Changjiang River estuary by single-gene-targeted metagenomics. 2014. |
入库方式： OAI收割
来源：海洋研究所
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