Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus(Selenka) juveniles exposed to nitrite
文献类型：CNKI期刊论文
| 作者 | Luo ZY(骆作勇); Wang BJ(王宝杰); Liu M(刘梅); Jiang KY(蒋克勇); Liu MX(刘明星); Wang L(王雷) |
| 发表日期 | 2014-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | sea cucumber Apostichopus japonicus Selenka vitamin C nitrite stress antioxidant enzyme reactive oxygen species(ROS) response surface methodology(RSM) |
| 英文摘要 | Different amounts of vitamin C were added to diets fed to juveniles(2.5 ± 0.15 g) of sea cucumber Apostichopus japonic u s(Selenka) in an attempt to reduce the stress response of specimens exposed to nitrite stress. A commercial feed was used as the control diet and three experimental diets were made by supplementing 1 000, 1 500, or 2 000 mg vitamin C/kg diet to control diet separately in a 45-day experiment. Sea cucumbers were exposed to three different levels(0.5, 1.0, and 1.5 mg/L) of nitrite stress for 4, 8, and 12 h at four time intervals(0, 15, 30, and 45 d). Growth of the animals was recorded during the experiment. Reactive oxygen species(ROS)(i.e. hydroxyl free radical(-OH), malondialdehyde(MDA) and total antioxidant capacity(T-AOC)) and antioxidant enzyme activities(i.e., superoxide dismutase(SOD) and catalase(CAT)) were measured. Response surface methodology(RSM) was used to analyze the effect of multiple factors on ROS indices and enzyme activities. Weight gain(WG) and special growth rate(SGR) of vitamin C supplementation groups were significantly higher than those of control group( P< 0.05). The levels of-OH and MDA increased under exposure time extending and nitrite concentration increasing, whereas T-AOC level decreased. SOD and CAT activities increased at 4 h and 8 h and decreased at 12 h. During the days in which the animal consumed experimental diets, the levels of-OH and MDA decreased and that of T-AOC increased. This result suggests that diets containing vitamin C could reduce the nitrite stress response in the animals and increase their antioxidant capacity. The multifactor regression equation of growth performance, ROS indices, and duration of feeding results suggest that vitamin C supplementation of 1 400–2 000 mg/kg diet for 29–35 days could reduce effectively the effects of nitrite exposure. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences(No.KZCX2-EWQ215) |
| 卷 | v.32期:04页:15-29 |
| 语种 | 英文; |
| 分类号 | S963 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190401]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.National&LocalJointEngineeringLaboratoryforEcologicalMariculture,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Luo ZY,Wang BJ,Liu M,et al. Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus(Selenka) juveniles exposed to nitrite. 2014. |
入库方式： OAI收割
来源：海洋研究所
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