Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus
文献类型：CNKI期刊论文
| 作者 | Xing MX(邢孟欣); Hou ZH(侯战辉); Qu YM(曲艳梅); Liu B(刘斌) |
| 发表日期 | 2014-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | turbot gastrointestinal supernatant 16S rRNA gene operational taxonomic units(OTUs) cultivation efficiency |
| 英文摘要 | Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot(Scophthalmus maximus),including 16 agar media with or without 1% gastrointestinal(GI)supernatant,or with 2% or 4% GI supernatant.A total of 1 711 colonies were analyzed and 24 operational taxonomic units(OTUs)were identifi ed.The greatest bacterial diversity was isolated on Zobell 2216E/Zobell 2216E+ agar media,whereas MRS/MRS+ agar media produced a low diversity of colonies.Agar media with GI supernatant(1%,2%,or 4%)showed increased diversity and yielded different profi les of OTUs from the corresponding original media,suggesting that GI supernatant provides substances that enhance the culture effi ciency of bacteria from the turbot GI tract.The large majority of the colonies(82%)were γ-Proteobacteria,whereas 15.6% and 2.4% of colonies were Firmicutes and Actinobacteria,respectively.At the genus level,49.4% of all colonies were assigned to Vibrio.Other potential pathogens,including Pseudomonas,Photobacterium,and Enterobacter,and potential probiotics,including Bacillus,Paenibacillus,and Pseudomonas,were also isolated on agar media.Most cultured bacteria belonged to species that were fi rst described in the turbot GI tract.The impact of these species on turbot physiology and health should be investigated further. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(No.2007AA09Z444) |
| 卷 | v.32期:02页:78-87 |
| 语种 | 英文; |
| 分类号 | R378 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190525]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Xing MX,Hou ZH,Qu YM,et al. Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus. 2014. |
入库方式： OAI收割
来源：海洋研究所
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