Characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica(Laminariales,Phaeophyta)
文献类型：CNKI期刊论文
| 作者 | Shao ZR(邵展茹); Liu FL(刘福利); Li QY(李秋莹) ; Yao JT(姚建亭); Duan DL(段德麟)
|
| 发表日期 | 2014-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Saccharina japonica Rubisco photosynthetic genes transcription protein expression carboxylase activity |
| 英文摘要 | Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments,and consequently may have highly effi cient ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)activity for carbon assimilation.In our study,we cloned the full-length Rubisco gene from S.japonica(SJ-rbc).It contained an open reading frame for a large subunit gene(SJ-rbcL)of 1 467 bp,a small subunit gene(SJ-rbcS)of 420 bp,and a SJ-rbcL /S intergenic spacer of 269 bp.The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa,5.81 and 15.84 kDa,4.71,respectively.After induction with 1 mmol/L isopropyl-β-Dthiogalactopyranoside for 5 h and purifi cation by Ni 2+ affi nity chromatography,electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein.Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night.This observation implied that Rubisco played a role in normal gametophytic growth and development.In juvenile sporophytes,mRNA levels of SJ-rbcL,carbonic anhydrase,Calvin-BensonBassham cycle-related enzyme,and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance.Similarly,expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m 2·s).Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Agriculture Science&Technology Achievements Transformation Fund(No.2011GB24910005) ; the Modern Agricultural-Industry Technology Research Project(No.200903030) ; the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A406) ; the Shandong Agriculture Breeding Engineering Biological Resources Innovation of Research Project ; the National“Twelfth Five-Year”Plan for Science&Technology Support(No.2013BAB01B01) |
| 卷 | v.32期:02页:139-151 |
| 语种 | 英文; |
| 分类号 | S511.22 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190528]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.YellowSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences 3.UniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Shao ZR,Liu FL,Li QY,et al. Characterization of ribulose-1,5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica(Laminariales,Phaeophyta). 2014. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。