A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis
文献类型：CNKI期刊论文
| 作者 | Wang JF(王家丰); Xu F(许飞); Li L(李莉); Zhang GF(张国范) |
| 发表日期 | 2014-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | identification cytochrome oxidase subunit I(COI) Crassostrea oyster high-resolution melting(HRM) |
| 英文摘要 | The high phenotypic plasticity in the shell of oysters presents a challenge during taxonomic and phylogenetic studies of these economically important bivalves.However,because DNA can exhibit marked differences among morphologically similar species,DNA barcoding offers a potential means for oyster identifi cation.We analyzed the complete sequences of the cytochrome oxidase subunit I(COI)of fi ve common Crassostrea species in China(including Hong Kong oyster C.hongkongensis,Jinjiang oyster C.ariakensis,Portuguese oyster C.angulata,Kumamoto oyster C.sikamea,and Pacifi c oyster C.gigas)and screened for distinct fragments.Using these distinct fragments on a high-resolution melting analysis platform,we developed an identifi cation method that does not rely on species-specifi c PCR or fragment length polymorphism and is effi cient,reliable,and easy to visualize.Using a single pair of primers(OysterCOI-1),we were able to successfully distinguish among the fi ve oyster species.This new method provides a simple and powerful tool for the identifi cation of oyster species. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China(973 Program)(No.2010CB126402) ; the National Natural Science Foundation of China(Nos.40730845,41206149) ; the Shandong Provincial Natural Science Foundation(No.ZR2010DQ024) ; the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A405) ; the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-48) ; the Taishan Scholar Program of Shandong Province ; the Taishan Scholar Climbing Program of Shandong Province |
| 卷 | v.32期:02页:181-187 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190529]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.UniversityofChineseAcademyofSciences 2.National&LocalJointEngineeringLaboratoryofEcologicalMariculture,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Wang JF,Xu F,Li L,et al. A new identification method for five species of oysters in genus Crassostrea from China based on high-resolution melting analysis. 2014. |
入库方式： OAI收割
来源：海洋研究所
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