Effect of Fe~(3+) on the growth and lipid content of Isochrysis galbana
文献类型：CNKI期刊论文
| 作者 | Liu ZY(刘志媛); Wang GC(王广策) |
| 发表日期 | 2014-01-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | biodiesel cell density iron microalgae Nile red |
| 英文摘要 | Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production of biodiesel from microalgae. In two experiments, we demonstrate that the cell concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I, adding chelated FeCl 3 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I. galbana cells. The fi nal cell density and lipid content of I. galbana supplemented with chelated FeCl 3 was approximately 2 and 1.65 times higher than that of non-supplemented cultures, respectively. In Experiment II, I. galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe 3+ at various concentrations. The fi nal cell concentration and lipid content were maximized at the highest iron concentration(38% biomass by dry weight at 1.2×10-5 mol/L Fe 3+). In this study, intracellular neutral lipid storage was evaluated by fl uorescent spectrophotometry using fl uorochrome Nile red, and the measurement conditions were optimized. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.40966002) ; the Natural Science Foundation of Hainan Province,China(No.409001) |
| 卷 | v.32期:01页:49-55 |
| 语种 | 英文; |
| 分类号 | S917.3 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190599]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.CollegeofOceanology,HainanUniversity |
| 推荐引用方式 GB/T 7714 | Liu ZY,Wang GC. Effect of Fe~(3+) on the growth and lipid content of Isochrysis galbana. 2014. |
入库方式： OAI收割
来源：海洋研究所
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