Purification and Identification of a Clotting Protein from the Hemolymph of Chinese Shrimp (Fenneropenaeus chinensis)
文献类型：CNKI期刊论文
| 作者 | WANG Baojie; PENG Hongni; LIU Mei; JIANG Keyong; ZHANG Guofan; WANG Lei |
| 发表日期 | 2013-09-06 |
| 出处 | Journal of Ocean University of China
 |
| 关键词 | Fenneropenaeus chinensis clotting protein purification proteomic identification MALDI-TOF/TOF MS |
| 英文摘要 | The clotting protein(CP) plays important and diverse roles in crustaceans,such as coagulation and lipid transportation.A clotting protein was purified from the hemolymph of Chinese shrimp Fenneropenaeus chinensis(named as Fc-CP) with Q sepharose HP anion-exchange chromatography and phenyl sepharose HP hydrophobic interaction chromatography.Fc-CP was able to form stable clots in vitro in the presence of hemocyte lysate and Ca2+,suggesting that the clotting reaction is catalyzed by a Ca2+-dependent transglutaminase in shrimp hemocytes.The molecular mass of Fc-CP was 380 kDa under non-reducing conditions and 190 kDa under reducing conditions as was determined with SDS-PAGE.CP exists as disulfide-linked homodimers and oligomers.The N-terminal amino acid sequence of Fc-CP was identical to that of shrimps including Penaeus monodon,Farfantepenaeus paulensis and Litopenaeus vannamei;and similar to that of other decapods.The purified Fc-CP was digested with trypsin and verified on an ABI 4700 matrix-assisted laser desorption/ionization tandem time-of-flight(MALDI-TOF/TOF) mass spectrometry.Our results will aid to better understanding the coagulation mechanism of shrimp hemolymph. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | supported by the National Natural Science Foundation of China(No.30600458) |
| 卷 | v.12期:03页:163-169 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 1672-5182 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190768]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanlogy,ChineseAcademyofSciences 2.GraduateSchoolofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | WANG Baojie,PENG Hongni,LIU Mei,et al. Purification and Identification of a Clotting Protein from the Hemolymph of Chinese Shrimp (Fenneropenaeus chinensis). 2013. |
入库方式： OAI收割
来源：海洋研究所
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