Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum
文献类型：CNKI期刊论文
| 作者 | LIU Yun; SONG Xiuxian ; HAN Xiaotian; YU Zhiming
|
| 发表日期 | 2013-06-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | high-affinity nitrate transporter gene, phosphate starvation, nitrate concentrations, real-time PCR, Skeletonema costatum |
| 英文摘要 | To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 μmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = 3.305x + 98.95, R2 = 0.988, where x represents nitrate concentrations (<40 μmol/L) and y represents the Nrt2 transcript levels. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The National Basic Research Program of China(973Program)under contract No.2010CB428706 ; the National Natural Science Foundation of China for Creative Research Groups under contract No.41121064 ; the National High Technology Research and Development Program of China under contract No.2008AA09Z107 |
| 卷 | v.32期:06页:84-90 |
| 语种 | 英文; |
| 分类号 | Q943 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190851]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateSchooloftheChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | LIU Yun,SONG Xiuxian,HAN Xiaotian,et al. Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum. 2013. |
入库方式： OAI收割
来源：海洋研究所
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