Peptidomic analysis of Chinese shrimp(Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDITOF MS
文献类型：CNKI期刊论文
| 作者 | Wang BJ(王宝杰); Liu M(刘梅); Jiang KY(蒋克勇); Zhang GF(张国范); Wang L(王雷) |
| 发表日期 | 2013-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Fenneropenaeus chinensis hemolymph peptidome MALDI-TOF MS WCX-magnetic bead immune-challenged |
| 英文摘要 | Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based purification(ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) to characterize shrimp hemolymph peptides.Shrimp serum and plasma were used as the source of samples for comparative analysis,and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis.To screen potential specific biomarkers in serum of immune-challenged shrimps,we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps.The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software.Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide(LPS)-infection.The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%.Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph,and will help to enable a better understanding of the innate immune response of shrimps. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China(No.30600458) |
| 卷 | v.31期:02页:163-171 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190932]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanlogy,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Wang BJ,Liu M,Jiang KY,et al. Peptidomic analysis of Chinese shrimp(Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDITOF MS. 2013. |
入库方式： OAI收割
来源：海洋研究所
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