Genetic transformation of marine cyanobacterium Synechococcus sp.CC9311(Cyanophyceae) by electroporation
文献类型：CNKI期刊论文
| 作者 | Chen HX(陈华新); Lin HZ(林瀚智); Jiang P(姜鹏); Li FC(李富超); Qin S(秦松) |
| 发表日期 | 2013-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | chromatic acclimation electroporation genetic transformation Synechococcus sp.CC9311 |
| 英文摘要 | Synechococcus sp.CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation(CA).A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA.The results show that Synechococcus sp.CC9311 cells were sensitive to four commonly used antibiotics:ampicillin,kanamycin,spectinomycin,and chloramphenicol.An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV,using a kanamycin resistance gene as selectable marker,was constructed by recombinant polymerase chain reaction.The plasmid was then transformed into Synechococcus sp.CC9311 via electroporation.High transformation efficiency was achieved at a field strength of 2 kV/cm.DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin.In addition,the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Key Innovation Project of Institute of Oceanology,Chinese Academy of Sciences(No.2009-2) ; the Natural Science Foundation of Shandong Province(No.2009ZRB02542) ; the Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques,SOA(No.MBSMAT-2010-03) ; the National Natural Science Foundation of China(No.41276164) ; the Natural Science Foundation of Jiangsu Province(No.BK2012650) |
| 卷 | v.31期:02页:172-176 |
| 语种 | 英文; |
| 分类号 | Q943 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190936]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences 3.YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Chen HX,Lin HZ,Jiang P,et al. Genetic transformation of marine cyanobacterium Synechococcus sp.CC9311(Cyanophyceae) by electroporation. 2013. |
入库方式： OAI收割
来源：海洋研究所
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