Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria
文献类型：CNKI期刊论文
| 作者 | SUN Yumiao; LI Fuhua; CHI Yanhong; XIANG Jianhai
|
| 发表日期 | 2013-02-15 |
| 出处 | Acta Oceanologica Sinica
 |
| 关键词 | WSSV, VP28, Exopalamon carincauda, live bacterial deliver |
| 英文摘要 | Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 ± 0.08, compared with 0.41 ± 0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | The National Basic Research Program of China (973 Program) under contract No. 2012CB114403 ; General Program of National Natural Science Foundation of China to Dr. Fuhua Li under contract No. 31072203 ; National Hightech Research and Development Program under contract Nos 2012AA10A404 and 2012AA092205 ; China Agriculture Research System under contract No. CARS-47 |
| 卷 | v.32期:02页:54-60 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0253-505X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/190969]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversity,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | SUN Yumiao,LI Fuhua,CHI Yanhong,et al. Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria. 2013. |
入库方式： OAI收割
来源：海洋研究所
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