Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus
文献类型：CNKI期刊论文
| 作者 | Jiang KY(蒋克勇); Sun SJ(孙姝娟); Liu M(刘梅); Wang BJ(王宝杰); Meng XL(孟晓林); Wang L(王雷) |
| 发表日期 | 2013-01-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | AMPD1 cDNA cloning mRNA expression IMP skeletal muscle Japanese flounder |
| 英文摘要 | AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5'-UTR of 78 bp, a 3'-UTR of 237 bp and an open reading frame (ORF) of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif (SLSTDDP) and an ATP-binding site sequence (EPLMEEYAIAAQVFK). Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle (750±40 g) compared with juvenile muscle (7.5±2 g) (P<0.05). HPLC analysis showed that the IMP content in adult muscle (3.35±0.21 mg/g) was also statistically significantly higher than in juvenile muscle (1.08±0.04 mg/g) (P<0.05). There is a direct relationship between the AMPD1 gene expression level and IMP content in the skeletal muscle of juvenile and adult flounders. These results may provide useful information for quality improvement and molecular breeding of aquatic animals. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China (No.41206144) ; the National High Technology Research and Development Program of China (863 Program) (No. 2008AA100805) |
| 卷 | v.31期:01页:120-129 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191017]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.R&DCenterofMarineBiotechnology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Jiang KY,Sun SJ,Liu M,et al. Cloning of the cDNA encoding adenosine 5'-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus. 2013. |
入库方式： OAI收割
来源：海洋研究所
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