Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish
文献类型：CNKI期刊论文
| 作者 | Wang DD(王冬冬); Li FH(李富花); Li SH(李诗豪); Wen R(温荣); Xiang JH(相建海)
|
| 发表日期 | 2012-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | antimicrobial peptides (AMPs) expression profiles bacteria Relish RNA interference |
| 英文摘要 | Antimicrobial peptides (AMPs), as key immune effectors, play important roles in the innate immune system of invertebrates. Different types of AMPs, including Penaeidin, Crustin, ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however, systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study, we analyzed the expression profiles of AMPs in the Chinese shrimps, Fenneropenaeus chinensis, simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive, G + ) or Vibrio anguillarium (Gram-negative, G+). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium, and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore, the expression of these AMPs showed temporal expression profiles, suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China (Nos.31072203, 30230280) ; the Special Fund for Agro-Scientific Research in the Public Interest (No. 201103034) ; the National Basic Research Program of China (973 Program) (No. 2012CB114403) |
| 卷 | v.30期:04页:107-115 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191186]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Wang DD,Li FH,Li SH,et al. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish. 2012. |
入库方式： OAI收割
来源：海洋研究所
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