Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5
文献类型：CNKI期刊论文
| 作者 | Cai JL(才金玲); Wei Y(魏莹); Zhao YP(赵宇鹏); Pan GH(潘光华); Wang GC(王广策) |
| 发表日期 | 2012-07-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | polyhydroxybutyrate Rhodovulum sulfidophilum nitrogen source carbon source carbon to nitrogen molar ratio |
| 英文摘要 | The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate (PHB) production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation (8.10±0.09 g/L) and PHB production (1.11±0.13 g/L and 14.62%±2.25% of the cell dry weight) were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH 4 + -N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration (7.65±0.05 g/L) was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB (5.03±0.18 g/L and 66.93%±1.69% of the cell dry weight) was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation (8.77±0.58 g/L) and PHB production (6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight) was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China (Nos.30830015, 40806063) ; the Key Natural Science Foundation of Tianjin,China (No. 12JC2DJC22200) ; the Natural Science Foundation of Guangxi,China (No. 1000050096) ; the Foundation of Tianjin Key Laboratory of Marine Resources and Chemistry (Tianjin University of Science & Technology) (No. 200913) ; the Introduced Talents Scientific Research Initiating Foundation of Tianjin University of Science and Technology (No.20100410) |
| 卷 | v.30期:04页:116-122 |
| 语种 | 英文; |
| 分类号 | X703 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191187]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.TianjinKeyLaboratoryofMarineResourcesandChemistry,CollegeofMarineScienceandEngineering,TianjinUniversityofScienceandTechnology 2.InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Cai JL,Wei Y,Zhao YP,et al. Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5. 2012. |
入库方式： OAI收割
来源：海洋研究所
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