Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)
文献类型：CNKI期刊论文
| 作者 | Xu N(徐娜); Pang SJ(逄少军); Dan TF(单体锋); Liu F(刘峰); Zhao XB(赵小波); Gao SQ(高素芹) |
| 发表日期 | 2012-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Eutreptiella microalgal bloom 18S rDNA 16S rDNA |
| 英文摘要 | During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic (18S rDNA and 16S rDNA sequences) analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20°C, 160 μmol photons/(m2×s) of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 μmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Special Research Fund for Non-Profit Sector(Agriculture) (No. 200903030) ; the National Key Basic Program of Science & Technology-Platforms of Aquaculture Stock Resources ; a Project from Chinese Academy of Sciences |
| 卷 | v.30期:03页:88-97 |
| 语种 | 英文; |
| 分类号 | Q94-3 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191254]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Xu N,Pang SJ,Dan TF,et al. Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae). 2012. |
入库方式： OAI收割
来源：海洋研究所
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