Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation
文献类型：CNKI期刊论文
| 作者 | Cui YL(崔玉琳); Jiang P(姜鹏); Wang JF(王金锋); Li FC(李富超); Chen YJ(陈英杰); Zheng GT(郑国庭); Qin S(秦松) |
| 发表日期 | 2012-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | bar glass-bead agitation nuclear transformation particle bombardment Platymonas subcordiformis |
| 英文摘要 | Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China (973 Program) (No. 2011CB200902) ; the National High Technology Research and Development Program of China (863 Program) (Nos. 2009AA10Z106,2009AA064401) ; the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. 2010BSB02009) ; the National Natural Science Foundation of China (Nos. 40876082,30870247) ; the Science and Technology Project of Qingdao City (No.09-1-3-59-jch) |
| 卷 | v.30期:03页:113-117 |
| 语种 | 英文; |
| 分类号 | Q943 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191255]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences 3.KeyLaboratoryofMarineBiotechnology,NingboUniversity |
| 推荐引用方式 GB/T 7714 | Cui YL,Jiang P,Wang JF,et al. Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation. 2012. |
入库方式： OAI收割
来源：海洋研究所
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