Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China
文献类型：CNKI期刊论文
| 作者 | Deng YY(邓蕴彦); Tang XR(汤晓荣); Huang BX(黄冰心); Teng LH(滕林宏); Ding LP(丁兰平) |
| 发表日期 | 2012-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Acrochaete leptochaete asexual reproduction culture observation growth characteristics molecular identification |
| 英文摘要 | Acrochaete leptochaete (Huber) Nielsen (Chaetophoraceae, Chlorophyta) was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21°C and a lower irradiance of 36 μmol/(m2·s) were most favorable for the growth of A. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species' general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Natural Science Foundation of China (Nos.31070185, 40876081 and 31093440) ; the Knowledge Innovation Program of Chinese Academy of Sciences (No. KSCX2-YW-Z-018) ; the Science and Technology Plan Project of Guangdong Province (No. 2011B031100010) ; the Team Project of Natural Science Foundation of Guangdong Province(No. S2011030005257) ; the Talent Introduction Projects of Guangdong Province Universities and Colleges ; the Grant of Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms, S.O.A., MATHAB (No. MATHAB20100301) ; the Science and Technology Plan Project of Shantou City (No. 2011-162) ; the Startup Projects (Nos. 09400133 and 09400134) ; the Grant for Youth Teachers of Shantou University, P. R. China |
| 卷 | v.30期:03页:118-126 |
| 语种 | 英文; |
| 分类号 | Q949 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191256]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences 3.MarineGeneticsandBreedingLaboratory,OceanUniversityofChina 4.ShantouUniversity |
| 推荐引用方式 GB/T 7714 | Deng YY,Tang XR,Huang BX,et al. Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China. 2012. |
入库方式： OAI收割
来源：海洋研究所
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