Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis
文献类型：CNKI期刊论文
| 作者 | Xiong ZF(熊志方); Li TG(李铁刚) ; Xavier Crosta
|
| 发表日期 | 2012-04-15 |
| 出处 | Journal of Earth Science
 |
| 关键词 | large diatom, stable isotope, physical separation, chemical oxidation, Parece Vela basin, palaeoceanography. |
| 英文摘要 | Diatom stable isotope analysis offers considerable potential in palaeoceanography, par-ticularly where carbonate material is scarce or absent. However, extracting pure diatom frustules free of external labile organic matter from marine sediments is an essential requirement for their applica-tions as paleoenvironmental proxies. Here, based largely on previous work, we developed a method in-cluding physical separation and chemical oxidation steps to concentrate and clean pure large diatoms from laminated diatom mat and diatomaceous clay sediment samples for their stable isotope analysis. Using the physical separation techniques consisting of the removal of carbonate and excess organic matter, sieving, differential settling, and heavy liquid floatation, pure diatoms can be successfully iso-lated from the sediment samples with opal concentration more than 10%. Subsequent time oxidation experiment shows that labile organic matter coating pure diatom valves can be effectively removed with 30% H2O2 at 65 ℃ for 2 h. Measurements of δ13C after every step of physical separation demonstrate that contaminants and lost diatoms can influence the original diatom stable isotope signal, highlighting the importance of a visual check for dominant diatom size in the initial sample and purity in the final sample. Although the protocol described here was only applied to diatom mats or diatom oozes con-taining large diatoms (Ethmodiscus rex), we believe that this method can be adapted to common dia-toms of general marine sediment samples. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | supported by the National Natural Science Foundation of China (No. 40776031) ; the National Fundamental Research and Development Planning Project (No. 2007CB815903) |
| 卷 | v.23期:02页:37-48 |
| 语种 | 英文; |
| 分类号 | P736.21 |
| ISSN号 | 1674-487X |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191298]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofMarineGeologyandEnvironment,InstituteofOceanology,ChineseAcademyofSciences 2.UMR-CNRS5805EPOC,UniversitéBordeaux1,AvenuedesFacultés |
| 推荐引用方式 GB/T 7714 | Xiong ZF,Li TG,Xavier Crosta. Cleaning of Marine Sediment Samples for Large Diatom Stable Isotope Analysis. 2012. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。