High genetic diversity in gametophyte clones of Undaria pinnatifida from Vladivostok, Dalian and Qingdao revealed using microsatellite analysis
文献类型：CNKI期刊论文
| 作者 | Dan TF(单体锋); Pang SJ(逄少军); Liu F(刘峰); Xu N(徐娜); Zhao XB(赵小波); Gao SQ(高素芹) |
| 发表日期 | 2012-03-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Undaria pinnatifida microsatellite analysis sex-linked locus genetic distance variety breeding |
| 英文摘要 | Breeding practice for Undaria pinnatifida (Harvey) Suringar requires the screening of a large number of offspring from gametophyte crossings to obtain an elite variety for large-scale cultivation. To better understand the genetic relationships of different gametophyte cultures isolated from different sources, 20 microsatellite loci were screened and 53 gametophyte clone cultures analyzed for U. pinnatifida isolated from wild sporophytes in Vladivostok, Russia and from cultivated sporophytes from Dalian and Qingdao, China. One locus was abandoned because of poor amplification. At the sex-linked locus of Up-AC-2A8, 3 alleles were detected in 25 female gametophyte clones, with sizes ranging from 307 to 316 bp. At other loci, 3 to 7 alleles were detected with an average of 4.5 alleles per locus. The average number of alleles at each locus was 1.3 and 3.7 for Russian and Chinese gametophyte clones, respectively. The average gene diversity for Russian, Chinese, and for the combined total of gametophyte clones was 0.1, 0.4, and 0.5, respectively. Russian gametophyte clones had unique alleles at 7 out of the 19 loci. In cluster analysis, Russian and Chinese gametophyte clones were separated into two different groups according to genetic distance. Overall, high genetic diversity was detected in gametophyte clones isolated from the two countries. These gametophyte cultures were believed to be appropriate parental materials for conducting breeding programs in the future. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Special Research Fund for Non-Profit Sector(Agriculture)(No.200903030) ; the Agriculture Science Technology Achievement Transformation Fund(No.2010GB24910699) ; the Knowledge Innovation Programs of Chinese Academy of Sciences(No.KSCX2-EW-B-3) |
| 卷 | v.30期:02页:35-40 |
| 语种 | 英文; |
| 分类号 | S917.3 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191330]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Dan TF,Pang SJ,Liu F,et al. High genetic diversity in gametophyte clones of Undaria pinnatifida from Vladivostok, Dalian and Qingdao revealed using microsatellite analysis. 2012. |
入库方式： OAI收割
来源：海洋研究所
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