Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3
文献类型：CNKI期刊论文
| 作者 | Ren SY(任世英); Li XQ(李相前); Jia JB(贾建波); Liu F(刘飞); Xiao T(肖天) |
| 发表日期 | 2011-09-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Halomonas sp. YSR-3 D-hydantoinase polyphosphate-accumulating bacterium |
| 英文摘要 | Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China (973 Program) (No. 2006AA10A401) ; the National Natural Science Foundation of China (No. 40376048) |
| 卷 | v.29期:05页:3-7 |
| 语种 | 英文; |
| 分类号 | Q93;Q78 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191571]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.SchoolofLifeScienceandChemicalEngineering,HuaiyinInstituteofTechnology 2.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Ren SY,Li XQ,Jia JB,et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3. 2011. |
入库方式： OAI收割
来源：海洋研究所
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