Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform
文献类型：CNKI期刊论文
| 作者 | Li N(李楠); Zhang L(张蕾); Li FC(李富超); Wang YZ(王玥珠); Zhu YQ(朱永强); Kang H(康慧); Wang SY(王升跃); Qin S(秦松) |
| 发表日期 | 2011-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Microcystis aeruginosa ectosymbiosis diversity COGs algal bloom metagenome |
| 英文摘要 | In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences, we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community: Archaea (n = 9; 0.01%), Bacteria (n = 98 921; 99.6%), Eukaryota (n = 373; 3.7%), and Viruses (n = 18; 0.02%). The bacterial sequences were predominantly Alphaproteobacteria (n = 41 805; 83.3%), Betaproteobacteria (n = 5 254; 10.5%) and Gammaproteobacteria (n = 1 180; 2.4%). Gene annotations and assignment of COG (clusters of orthologous groups) functional categories indicate that a large number of the predicted genes are involved in metabolic, genetic, and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No. KSCX2-YW-G-073) |
| 卷 | v.29期:03页:15-23 |
| 语种 | 英文; |
| 分类号 | Q948.8 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191704]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences 2.InstituteofOceanology,ChineseAcademyofSciences 3.GraduateUniversityofChineseAcademyofSciences 4.SouthChinaSeaInstituteofOceanology,ChineseAcademyofSciences 5.Shanghai-MOSTKeyLaboratoryofHealthandDiseaseGenomics,ChineseNationalHumanGenomeCenteratShanghai |
| 推荐引用方式 GB/T 7714 | Li N,Zhang L,Li FC,et al. Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform. 2011. |
入库方式： OAI收割
来源：海洋研究所
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