中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform

文献类型:CNKI期刊论文

作者Li N(李楠); Zhang L(张蕾); Li FC(李富超); Wang YZ(王玥珠); Zhu YQ(朱永强); Kang H(康慧); Wang SY(王升跃); Qin S(秦松)
发表日期2011-05-15
出处Chinese Journal of Oceanology and Limnology
关键词Microcystis aeruginosa ectosymbiosis diversity COGs algal bloom metagenome
英文摘要In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences, we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community: Archaea (n = 9; 0.01%), Bacteria (n = 98 921; 99.6%), Eukaryota (n = 373; 3.7%), and Viruses (n = 18; 0.02%). The bacterial sequences were predominantly Alphaproteobacteria (n = 41 805; 83.3%), Betaproteobacteria (n = 5 254; 10.5%) and Gammaproteobacteria (n = 1 180; 2.4%). Gene annotations and assignment of COG (clusters of orthologous groups) functional categories indicate that a large number of the predicted genes are involved in metabolic, genetic, and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing.
文献子类CNKI期刊论文
资助机构Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No. KSCX2-YW-G-073)
v.29期:03页:15-23
语种英文;
分类号Q948.8
ISSN号0254-4059
源URL[http://ir.qdio.ac.cn/handle/337002/191704]  
专题中国科学院海洋研究所
作者单位1.YantaiInstituteofCoastalZoneResearch,ChineseAcademyofSciences
2.InstituteofOceanology,ChineseAcademyofSciences
3.GraduateUniversityofChineseAcademyofSciences
4.SouthChinaSeaInstituteofOceanology,ChineseAcademyofSciences
5.Shanghai-MOSTKeyLaboratoryofHealthandDiseaseGenomics,ChineseNationalHumanGenomeCenteratShanghai
推荐引用方式
GB/T 7714
Li N,Zhang L,Li FC,et al. Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform. 2011.

入库方式: OAI收割

来源:海洋研究所

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