DNA barcoding discriminates Pampus minor(Liu et al.,1998) from Pampus species
文献类型：CNKI期刊论文
| 作者 | Guo EM(郭恩棉); Liu Y(刘媛); Liu J(刘静); Cui CX(崔朝霞) |
| 发表日期 | 2010-11-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Pampus minor COI 16S DNA barcoding |
| 英文摘要 | Although Pampus minor has been classified as a new species,it still remains controversial.Was used a DNA barcoding technique based on homologous sequence analysis of the16S and COI genes to clarify the confusion over the identification of this species.Among 12 individuals whose genetic distance was 0.002,two haplotypes were found.According to the 16S sequences,the genetic distances ranged from 0.121 to 0.133 between P.minor and other Pampus species.Although the same the genetic distance between the two P.minor haplotypes was generated using COI sequences,the haplotype of Pm22-23,Pm28,and Pm32-33 was the same as that of Pci EF607462 and EF607466,while the haplotype of Pm24-27 and Pm29-31 was the same as that of Pci EF607461 and EF607463-65.In addition,the genetic distance ranged only from 0.002 to 0.005 between P.minor and Pa EF607460 and EF607458.Apart from this,the interspecies genetic distances varied from 0.135 to 0.143 between P.minor and other Pampus species according to the COI sequences.Phylogenetic trees,using combined 16S and COI data,strongly support the viewpoint that all the P.minor individuals form one clade that is in a sister position to Pampus sp.individuals(EU357803,FJ434342-FJ434343,and FJ652423-FJ652427). |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by National Natural Science Foundation of China (No. 40676085) |
| 卷 | v.28期:06页:142-150 |
| 语种 | 英文; |
| 分类号 | Q943 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/191882]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.QingdaoAgricultureUniversity 2.InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Guo EM,Liu Y,Liu J,et al. DNA barcoding discriminates Pampus minor(Liu et al.,1998) from Pampus species. 2010. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。