PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp.on intestinal microbial diversity in kuruma shrimp(Marsupenaeus japonicus)
文献类型：CNKI期刊论文
| 作者 | Liu HD(刘淮德); Liu M(刘梅); Wang BJ(王宝杰); Jiang KY(蒋克勇); Jiang S(姜珊); Sun SJ(孙姝娟); Wang L(王雷) |
| 发表日期 | 2010-08-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | intestinal microbial diversity PCR-DGGE Kuruma shrimp |
| 英文摘要 | In this study,the intestinal microbiota of kuruma shrimp(Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp.on intestinal microbial diversity.Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp.amended feed.PCR and denaturing gradient gel electrophoresis(DGGE) analyses were then performed on DNA extracted directly from the guts.Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons,and distinct bands in the gels were sequenced.The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp.and uncultured gamma proteobacterium.Overall,the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2006AA100311,2008AA100805) |
| 卷 | v.28期:04页:98-104 |
| 语种 | 英文; |
| 分类号 | Q938 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192000]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.GraduateSchooloftheChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Liu HD,Liu M,Wang BJ,et al. PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp.on intestinal microbial diversity in kuruma shrimp(Marsupenaeus japonicus). 2010. |
入库方式： OAI收割
来源：海洋研究所
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