Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization
文献类型：CNKI期刊论文
| 作者 | Zhang BY(张宝玉); Chen GF(陈国福); Wang GC(王广策); Lu DD(陆斗定) |
| 发表日期 | 2010-04-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | fluorescent in situ hybridization ITS sequence Skeletonema costatum harmful algal bloom |
| 英文摘要 | A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2 from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including 5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising tool for detecting target HAB species in natural environments. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the High Technology Research and Development Program of China (863 Program) (2007AA09Z110) |
| 卷 | v.28期:02页:112-116 |
| 语种 | 英文; |
| 分类号 | Q943 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192160]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.TheInstituteofOceanology,ChineseAcademyofSciences 2.TheSecondInstituteofOceanography,SOA 3.TianjinUniversityofScienceandTechnology 4.HarbinInstituteofTechnology |
| 推荐引用方式 GB/T 7714 | Zhang BY,Chen GF,Wang GC,et al. Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization. 2010. |
入库方式： OAI收割
来源：海洋研究所
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