Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis):a chromosome-specific marker for chromosome identification
文献类型：CNKI期刊论文
| 作者 | Xun P(郇聘) ; Zhang XJ(张晓军); Li FH(李富花); Zhao C(赵翠); Zhang CS(张成松); Xiang JH(相建海)
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| 发表日期 | 2010-04-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Fenneropenaeus chinensis 5S rDNA chromosomal localization fluorescence in-situ hybridization(FISH) |
| 英文摘要 | Chinese shrimp(Fenneropenaeus chinensis)is an economically important aquaculture species in China.However,cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze.In this study,fluorescence in-situ hybridization(FISH) was used to identify the chromosomes of F.chinensis.The 5S ribosomal RNA gene(rDNA)of F. chinensis was isolated,cloned and then used as a hybridization probe.The results show that the 5S rDNA was located on one pair of homologous chromosomes in F.chinensis.In addition,triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method.It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F.chinensis.The successful application of FISH in F.chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Key Program of the National Natural ScienceFoundation of China(No.30730071) ; the National High-Tech Researchand Development Program of China(863 program)(No.2007AA09Z430) ; the General Program of National Natural Science Foundation of China(Nos.30471347&40706048) |
| 卷 | v.28期:02页:35-40 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192172]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Xun P,Zhang XJ,Li FH,et al. Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis):a chromosome-specific marker for chromosome identification. 2010. |
入库方式： OAI收割
来源：海洋研究所
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