Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease
文献类型：CNKI期刊论文
| 作者 | Mo ZL(莫照兰); Guo DS(郭东升); Mao YX(茅云翔); Ye XH(叶旭红); Zou YX(邹玉霞); Xiao P(肖鹏); Hao B(郝斌) |
| 发表日期 | 2010-02-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Vibrio anguillarum prtV proteolysis hemolysis mucus LD50 |
| 英文摘要 | We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In addition,the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes.Infection experiments showed that the LD50 of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish.We propose that prtV plays an important role in the pathogenesis of V.anguillarum. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China (973 Program) (No. 2006CB101803) ; the National Natural Science Foundation of China (No. 30871935) ; the National High Technology Research and Development Program (863 Program) (No. 2003AA622070) |
| 卷 | v.28期:01页:57-63 |
| 语种 | 英文; |
| 分类号 | S941 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192261]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.GraduateUniversityofChineseAcademyofSciences 2.InstituteofOceanology,ChineseAcademyofSciences 3.OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Mo ZL,Guo DS,Mao YX,et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease. 2010. |
入库方式： OAI收割
来源：海洋研究所
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