Simultaneous enumeration of diatom,protozoa and meiobenthos from marine sediments using Ludox-QPS method
文献类型：CNKI期刊论文
| 作者 | Du YF(杜永芬); Xu KD(徐奎栋) ; Lei YL(类彦立)
|
| 发表日期 | 2009-11-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | enumeration extraction meiobenthos microbenthos Ludox HS 40 centrifugation quantitative protargol stain |
| 英文摘要 | The Ludox-QPS method is a newly developed technique,which combines the Ludox HS 40 density centrifugation and quantitative protargol stain,to enumerate marine ciliates with good taxonomic resolution.We tested the method for simultaneous enumeration of diatoms,protozoa and meiobenthos and compared its extraction efficiency for meiobenthos with that of the routine Ludox-TM centrifugation and a modified protocol using Ludox HS 40.We conducted the evaluation with a sample size of 8.3 ml each from sandy,muddy-sand and muddy sediments collected from the intertidal area of the Yellow Sea in summer 2006 and spring 2007.The Ludox-QPS method not only produced high extraction efficiencies of 97±1.3% for diatoms and 97.6±0.8% for ciliates,indicating a reliable enumeration for eukaryotic microbenthos,but also produced excellent extraction efficiencies of on average 97.3% for total meiobenthos,97.9% for nematodes and 97.8% for copepods from sands,muddy sands and mud.By contrast,the routine Ludox-TM centrifugation obtained only about 74% of total meiobenthos abundance with one extraction cycle,and the modified Ludox HS 40 centrifugation yielded on average 93% of total meiobenthos:89.4±2.0% from sands,93±4.1% from muddy sands and 97.1±3.0% from mud.Apart from the sediment type,sample volume was another important factor affecting the extraction efficiency for meiobenthos.The extraction rate was increased to about 96.4% when using the same modified Ludox centrifugation for a 4 ml sediment sample.Besides the excellent extraction efficiency,the Ludox-QPS method obtained higher abundances of meiobenthos,in particular nematodes,than the routine Ludox centrifugation,which frequently resulted in an uncertain loss of small meiobenthos during the sieving process.Statistical analyses demonstrated that there were no significant differences between the meiobenthos communities revealed by the Ludox-QPS method and the modified Ludox HS 40 centrifugation,showing the high efficiency of the Ludox-QPS method for simultaneous enumeration of diatom,protozoa and meiobenthos.Moreover,the comparatively high taxonomic resolution of the method,especially for diatoms and ciliates,makes it feasible to investigate microbial ecology at community level. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No.KZCX2-YW-417) ; the National Natural Science Foundation of China (No.40576072 ; 40706047) ; the “100 Talents Project” of the Chinese Academy of Sciences |
| 卷 | v.27期:04页:81-89 |
| 语种 | 英文; |
| 分类号 | Q178.53 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192369]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.GraduateSchool,ChineseAcademyofSciences 2.InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Du YF,Xu KD,Lei YL. Simultaneous enumeration of diatom,protozoa and meiobenthos from marine sediments using Ludox-QPS method. 2009. |
入库方式： OAI收割
来源：海洋研究所
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