Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye
文献类型：CNKI期刊论文
| 作者 | Feng JH(冯金华); Yu HH(于华华) ; Li CP(李翠萍); Xing RE(邢荣娥); Liu S(刘松); Wang L(王琳); Cai SB(蔡圣宝); Li PC(李鹏程)
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| 发表日期 | 2009-11-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
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| 关键词 | nematocyst jellyfish venom Rhopilema esculentum hemolytic activity |
| 英文摘要 | The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye.The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry.Four types of nematocysts were identified:mastigophores,euryteles,and atrichous and holotrichous isorhiza.SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa,and that glutamic acid was the main amino acid.A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L,pH 7.8,5% glycerol,0.5 mmol/L EDTA,0.1 mol/L NaCl).The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by Qingdao Science and Technology Project (No. 08-1-3-51- JCH) ; the Award Foundation of Scientific Research for Excellent Young and Middle-Age Scientist of Shandong Province (No.2006BS 07003) |
| 卷 | v.27期:04页:175-180 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192382]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.GraduateSchool,ChineseAcademyofSciences 2.InstituteofOceanology,ChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Feng JH,Yu HH,Li CP,et al. Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye. 2009. |
入库方式： OAI收割
来源：海洋研究所
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