Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
文献类型：CNKI期刊论文
| 作者 | Niu JF(牛建峰); Wang GC(王广策); Lv F(吕芳); Zhou BC(周百成); Peng G(彭光) |
| 发表日期 | 2009-08-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Bryopsis hypnoides lectin hemagglutinating cell organelle aggregation regeneration |
| 英文摘要 | A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was not a glycoprotein.The hemagglutinating activity(HA) of the lectin was not dependent on the presence of divalent cations and was inhibited by N-Acetylgalactosamine,N-Acetylglucosamine,and the glycoprotein bovine submaxillary mucin.The lectin preferentially agglutinated Gram-negative bacterium.The HA of this lectin was stable between pH 4 to pH 10.Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution(0.5 mg ml-1).Our results suggest that the regeneration of B.hypnoides is mediated by this lectin.We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater(pH 8.0).Given that nigericin dissipates proton gradients across the membrane,we hypothesize that the aggregation of cell organelles was proton-gradient dependent. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Natural Science Foundation of China (Nos 40806063,30830015) ; the National High Technology Research and Development Program of China (863 Program) (Nos2007AA09Z406,2006AA10A413) |
| 卷 | v.27期:03页:84-94 |
| 语种 | 英文; |
| 分类号 | Q948 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192484]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.CollegeofMarineScienceandEngineering,TianjinUniversityofScienceandTechnology 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.TheGraduateSchoolofChineseAcademyofSciences |
| 推荐引用方式 GB/T 7714 | Niu JF,Wang GC,Lv F,et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides. 2009. |
入库方式： OAI收割
来源：海洋研究所
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