Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu
文献类型：CNKI期刊论文
| 作者 | Zhao WH(赵卫红); Wang JT(王江涛); Chen MM(陈玫玫) |
| 发表日期 | 2009-08-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | dissolved organic matter fluorescence excitation-emission matrix spectrum Prorocentrum donghaiense Lu |
| 英文摘要 | Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth,stationary and decline stages into <0.45 μm filtrate,100 kDa-0.45 μm,10-100 kDa and 1-10 kDa retentate and <1 kDa ultrafiltrate fractions.The fluorescence properties of different molecular weights of dissolved organic matter(DOM) were measured by excitation-emission matrix spectra.Protein-like and humic-like fluorophores were observed in the DOM produced by P.donghaiense.The central positions of protein-like fluorophores showed a red shift with prolonged growth duration,shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages.The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size,but showed little change in the decline stage.However,the emission wavelengths in the decline stage exhibited a blue shift.Very distinct C type and A type peaks in humic-like fluorophores were observed.With a prolonged culture time,the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift,while the A:C ratios fell.More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the High Technology Research and Development Program of China (863 Program) (Nos 2006AA09Z180 and 2004AA639790) ; the National Natural Science Foundation of China (No 40106013) ; the National Basic Research Program of China (973 Program) (No 2001CB409703) |
| 卷 | v.27期:03页:146-151 |
| 语种 | 英文; |
| 分类号 | Q945 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192486]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.KeyLaboratoryofEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.KeyLaboratoryofMarineChemistryTheoryandTechnology,MinistryofEducation,OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Zhao WH,Wang JT,Chen MM. Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu. 2009. |
入库方式： OAI收割
来源：海洋研究所
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