中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum

文献类型:CNKI期刊论文

作者Han YF(韩一凡); Mo ZL(莫照兰); Mao YX(茅云翔); Xiao P(肖鹏); Li J(李杰); Hao B(郝斌); Guo DS(郭东升)
发表日期2009-08-15
出处Chinese Journal of Oceanology and Limnology
关键词end-sequencing fosmid library PCR screening Vibrio anguillarum
英文摘要Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58(62.4%) were homologous to known genes,30(32.3%) were genes with hypothetical functions,and the remaining 5(5.3%) were unknown genes.We demonstrated the utility of this library by PCR screening of 10 genes.This resulted in an average of 6.2 fosmid clones per screening.This fosmid library offers a new tool for gene screening and cloning of V.anguillarum,and for comparative genomic studies among Vibrio species.
文献子类CNKI期刊论文
资助机构Supported by the National Basic Research Program of China (973 Program)(No 2006CB101803) ; the High Technology Research and development Program of China (863 Program)(No 2006AA6100310) ; the National Natural Science Foundation of China (No 30871935)
v.27期:03页:101-108
语种英文;
分类号S941
ISSN号0254-4059
源URL[http://ir.qdio.ac.cn/handle/337002/192498]  
专题中国科学院海洋研究所
作者单位1.InstituteofOceanology,ChineseAcademyofSciences
2.OceanUniversityofChina
推荐引用方式
GB/T 7714
Han YF,Mo ZL,Mao YX,et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum. 2009.

入库方式: OAI收割

来源:海洋研究所

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