Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum
文献类型：CNKI期刊论文
| 作者 | Han YF(韩一凡); Mo ZL(莫照兰); Mao YX(茅云翔); Xiao P(肖鹏); Li J(李杰); Hao B(郝斌); Guo DS(郭东升) |
| 发表日期 | 2009-08-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | end-sequencing fosmid library PCR screening Vibrio anguillarum |
| 英文摘要 | Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58(62.4%) were homologous to known genes,30(32.3%) were genes with hypothetical functions,and the remaining 5(5.3%) were unknown genes.We demonstrated the utility of this library by PCR screening of 10 genes.This resulted in an average of 6.2 fosmid clones per screening.This fosmid library offers a new tool for gene screening and cloning of V.anguillarum,and for comparative genomic studies among Vibrio species. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National Basic Research Program of China (973 Program)(No 2006CB101803) ; the High Technology Research and development Program of China (863 Program)(No 2006AA6100310) ; the National Natural Science Foundation of China (No 30871935) |
| 卷 | v.27期:03页:101-108 |
| 语种 | 英文; |
| 分类号 | S941 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192498]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Han YF,Mo ZL,Mao YX,et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum. 2009. |
入库方式： OAI收割
来源：海洋研究所
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