An improved PCR method for direct identification of Porphyra(Bangiales,Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer
文献类型：CNKI期刊论文
| 作者 | Wang C(王超); Dong D(董栋); Wang GC(王广策); Zhang BY(张宝玉); Peng G(彭光); Xu P(许璞); Tang XR(汤晓荣) |
| 发表日期 | 2009-08-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | Rhodophyta Porphyra RUBISCO intergenic spacer DNA sequence PCR |
| 英文摘要 | An improved method of PCR in which the small segment of conchocelis is amplified directly without DNA extraction was used to amplify a RUBISCO intergenic spacer DNA fragment from nine species of red algal genus Porphyra(Bangiales,Rhodophyta),including Porphyra yezoensis(Jiangsu,China),P.haitanensis(Fujian,China),P.oligospermatangia(Qingdao,China),P.katadai(Qingdao,China),P.tenera(Qingdao,China),P.suborboculata(Fujian,China),P.pseudolinearis(Kogendo,Korea),P.linearis(Devon,England),and P.fallax(Seattle,USA).Standard PCR and the method developed here were both conducted using primers specific for the RUBISCO spacer region,after which the two PCR products were sequenced.The sequencing data of the amplicons obtained using both methods were identical,suggesting that the improved PCR method was functional.These findings indicate that the method developed here may be useful for the rapid identification of species of Porphyra in a germplasm bank.In addition,a phylogenetic tree was constructed using the RUBISCO spacer and partial rbcS sequence,and the results were in concordant with possible alternative phylogenies based on traditional morphological taxonomic characteristics,indicating that the RUBISCO spacer is a useful region for phylogenetic studies. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the National High Technology Research and Development Program of China (863 Program)(No 2006AA10A402) ; Project for Supporting National Development (No 2006BAD09A04) ; the National Natural Science Foundation of China (Nos U0633006,40476059) ; the Natural Science Foundation of Qingdao (No 05-2-p-2) ; the Knowledge Innovation Program of the Chinese Academy of Sciences (No KZCX2-211) |
| 卷 | v.27期:03页:95-100 |
| 语种 | 英文; |
| 分类号 | S917.3 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192500]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.ChangshuInstituteofTechnology 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences 3.GraduateUniversityofChineseAcademyofSciences 4.TianjinUniversityofScienceandTechnology 5.OceanUniversityofChina |
| 推荐引用方式 GB/T 7714 | Wang C,Dong D,Wang GC,et al. An improved PCR method for direct identification of Porphyra(Bangiales,Rhodophyta) using conchocelis based on a RUBISCO intergenic spacer. 2009. |
入库方式： OAI收割
来源：海洋研究所
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